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ผลของชนิดของอิมัลซิไฟเออร์ต่อคุณลักษณะทางกายภาพของเบต้าแคโรทีนนาโนอิมัลชัน |
|---|---|
| รหัสดีโอไอ | |
| Creator | มนัส ชัยจันทร์ |
| Title | ผลของชนิดของอิมัลซิไฟเออร์ต่อคุณลักษณะทางกายภาพของเบต้าแคโรทีนนาโนอิมัลชัน |
| Contributor | เกศินี ศรีรัตนโชติ, วรวรรณ พันพิพัฒน์, หมุดตอเล็บ หนิสอ |
| Publisher | Thammasat University |
| Publication Year | 2562 |
| Journal Title | Thai Journal of Science and Technology |
| Journal Vol. | 8 |
| Journal No. | 6 |
| Page no. | 620-632 |
| Keyword | beta-carotene, emulsifier, nanoemulsion, physical property |
| URL Website | https://www.tci-thaijo.org/ |
| Website title | THAIJO |
| ISSN | 2286-7333 |
| Abstract | Preparation of beta-carotene in nanoemulsion is one of an effective means to improve beta-carotene bioactivity and bioavailability. The aim of this study was to investigate the effect of emulsifier types on the physical properties of beta-carotene nanoemulsion. To prepare the nanoemulsion, beta-carotene was dissolved in rice bran oil (45 percent w/w) at 60 degree Celcius under nitrogen gas for 1 h with oil volume fraction of 0.1. Different emulsifiers and their mixtures (3 percent) were employed for nanoemulsion fabrication including Tween 80, Span 80, lecithin, beta-lactoglobulin (beta-Lg), whey protein isolate (WPI), lecithin+Tween 80, lecithin+WPI and Tween 80+Span 80. Emulsion was prepared using a bath sonicator for 10 min followed by a probe sonicator (40 watt and 25 percent amplitude) for 10 min. The median droplet size diameter, polydispersity index (PDI), zeta potential, emulsion activity index (EAI), emulsion stability index (ESI) and microstructure of corresponded emulsions were immediately analyzed. The smallest mean particle size diameter (263 nm) was observed in nanoemulsion made by Tween 80 mixed with Span 80. This mixed emulsifier rendered the nanoemulsion with the lowest PDI and distributed more uniform when observed with confocal scanning light microscopy. All emulsions showed the zeta potential lower than -30 mV indicating high stability of these emulsions. The EAI and ESI were higher in proteins-stabilized emulsions. From the results, the beta-carotene nanoemulsion was successfully produced by the aid of Tween 80 mixed with Span 80 as an active emulsifier mixture, which exhibited the mean particle size diameter of less than 500 nm. |
