Effect of Planting Material and Spore Preservation Temperature of Astraeus odoratus on the Growth and Ectomycorrhizal Root Formation in Dipterocarpus alatus seedlings
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Creator Thapana Chabthaisong
Title Effect of Planting Material and Spore Preservation Temperature of Astraeus odoratus on the Growth and Ectomycorrhizal Root Formation in Dipterocarpus alatus seedlings
Contributor Tharnrat Kaewgrajang
Publisher Kasetsart University
Publication Year 2564
Journal Title Thai Journal of Forestry
Journal Vol. 40
Journal No. 2
Page no. 83-94
Keyword Relative growth rate, Mycorrhiza, Dipterocarpaceae, Spore suspension
URL Website https://li01.tci-thaijo.org/index.php/tjf/article/view/251056
Website title Thai Journal of Forestry
ISSN 2730-2180
Abstract The objectives of this study were to study the effect of planting materials and temperature on the preservation of Astraeus odoratus spores and their ability to grow and form ectomycorrhizal (ECM) root in Dipterocarpus alatus seedlings. Two formulae of planting materials included Formula 1 consisting of vermiculite + peat moss (8:2); and Formula 2 consisting of soil + granules black rice husk + coconut husk (1:1:1), were used in the experiment. The result indicated that seedlings inoculated with Formula 1 formed ECM colonization at a rate of 42.09%, which was not observed with Formula 2. Moreover, a relative growth rate of height was observed which was significantly higher than that in seedlings inoculated with Formula 2. To study the effect of temperature on preservation of A. odoratus spores, we preserved the spores for two years under three different temperatures including room temperature, 4 ฐC, and -20 ฐC. The result indicated that a 2-year-old preserved spore can be used as an ECM inoculum. The appropriate temperature for preservation spore was -20 ฐC. In addition, the inoculated seedlings with spores preserved at -20 ฐC showed a significantly higher ECM colonization rate (61.04%) than the inoculated seedlings with spores preserved at room temperature (22.51%).
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