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Genetic Relationships Analysis of Lime and Acid Citrus by ISSR Markers |
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| รหัสดีโอไอ | |
| Creator | Paweena Chuenwarin |
| Title | Genetic Relationships Analysis of Lime and Acid Citrus by ISSR Markers |
| Contributor | Sukawat Thongleow, Anyamanee Auvuchanon |
| Publisher | Department of Agriculture |
| Publication Year | 2565 |
| Journal Title | Thai Agricultural Research Journal |
| Journal Vol. | 40 |
| Journal No. | 2 |
| Page no. | 188-199 |
| Keyword | Mexican lime, Citrus, DNA markers, ISSR |
| URL Website | https://at.doa.go.th/journal |
| Website title | Thai Agricultural Research Journal |
| ISSN | 0125-8389 |
| Abstract | The major commercial lime cultivars in Thailand are Mexican lime (Citrus aurantifolia (Christm.) Swingle) and Tahiti lime (C. latifolia (Yu. Tanaka) Tanaka). These cultivars are either not resistant to diseases or the fruit quality do not meet the market demand. To breed for new cultivars with improved disease resistance and quality, lime and acid Citrus germplasms are the sources of parental lines. Information on genetic relationship between lime and acid Citrus germplasms will be very helpful for parental lines selection. This study aims to study the genetic relationship of 55 accessions of lime and acid Citrus germplasms by using Inter Simple Sequence Repeat (ISSR) markers. Four primers of ISSR generated 33 DNA bands with 87.5% polymorphism. Dendrogram revealed the ability of 4 ISSR primers to cluster lime and acid Citrus into four groups, a group of Mexican lime and Tahiti lime, a group of citron and lemon, a group of kaffir lime, and a group of finger lime which coincide with the classification of lime and acid Citrus to their species. Focusing on 26 accessions of Mexican lime, ISSR primer 814 could separate Mexican lime into two groups. Group A contained 13 accessions in which the average fruit weight, fruit rind thickness, and seed number were all less than group B which also contained 13 accessions. |
