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Mass production of the entomopathogenic nematode, Steinernema siamkayai EPNKU70 using artificial media |
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| รหัสดีโอไอ | |
| Creator | Atirach Noosidum |
| Title | Mass production of the entomopathogenic nematode, Steinernema siamkayai EPNKU70 using artificial media |
| Contributor | Phanuruk Pratubkong, Prakai Rajnuwong, Chatchaloem Arkhan, Kwanrudee Kachanan, Rattanawadee Onwong |
| Publisher | Faculty of Agriculture |
| Publication Year | 2568 |
| Journal Title | Khon Kaen Agriculture Journal |
| Journal Vol. | 53 |
| Journal No. | 4 |
| Page no. | 843-858 |
| Keyword | biological control, insect pathogen, insect pest, in vitro culture |
| URL Website | https://li01.tci-thaijo.org/index.php/agkasetkaj |
| Website title | Khon Kaen Agriculture Journal |
| ISSN | 3027-6497 (Online) |
| Abstract | Entomopathogenic nematodes (EPNs) are highly promising biocontrol agents for insect pest management. Their application in agricultural production systems necessitates large-scale mass production to reduce costs. This study aimed to investigate three artificial media, including liver-kidney agar, lipid agar, and a basal medium with soybean residue, to mass-produce Steinernema siamkayai EPNKU70 under laboratory conditions. The results showed that neither the basal medium with soybean residue nor the control treatment (agar only) yielded infective juveniles (IJs) in subsequent generations. Conversely, lipid agar demonstrated greater performance compared to liver-kidney agar for the propagation of S. siamkayai EPNKU70. Lipid agar and liver-kidney agar yielded 25,258.00 and 6,133.93 IJs/mL, respectively. The recovery times for lipid agar and liver-kidney agar were 3.17 and 3.23 days, respectively, while the developmental times to the next generation of IJs were 5.20 and 6.43 days, respectively. The total production periods were 12.13 and 16.63 days, respectively, with survival rates of 99.02 and 95.77%, respectively. The efficacy of IJs produced on Galleria mellonella L. larvae, lipid agar, and liver-kidney agar, was evaluated against the final instar G. mellonella. Results indicated no significant differences in infection rates across the treatments within 72 hours, with mortality rates of 100.00, 95.56, and 93.33%, respectively. These findings suggest that lipid agar is a highly effective medium for the mass production of S. siamkayai EPNKU70. |
