|
In Vitro Propagation of Heracleum siamicum Craib |
|---|---|
| รหัสดีโอไอ | |
| Creator | Patcharawadee Wattanawikkit |
| Title | In Vitro Propagation of Heracleum siamicum Craib |
| Contributor | Pattharaporn Srisamatthakarn |
| Publisher | Chiang Mai University |
| Publication Year | 2568 |
| Journal Title | Journal of Agricultural Research and Communications |
| Journal Vol. | 41 |
| Journal No. | 1 |
| Page no. | 23-34 |
| Keyword | Tissue culture, H. siamicum Craib., growth regulator |
| URL Website | https://li01.tci-thaijo.org/index.php/joacmu/article/view/264250 |
| Website title | Journal of Agricultural Research and Communications |
| ISSN | 3057-1022 |
| Abstract | Heracleum siamicum Craib. is herbal spice plant that contains many important chemical components. However, there are a few research information on its propagation by tissue culture. This research was aimed to study the optimal media for H. siamicum Craib. shoot induction. The 1 cm shoot tips were cultured on the MS and WPM media which supplemented with different of BAP and TDZ at concentrations of 0, 1, 2, 4 and 8 mg/L with 20 replicates for 45 days. The results showed that the BAP addition of any 2 mg/L gave significantly greater shoot number (5.55?0.87 shoots/explant) than the other (P<0.05). While, the MS and WPM media without growth regulators exhibited the greatest shoot length of 6.80?0.42 and 7.21?0.35 cm, respectively. The H. siamicum Craib. root induction was afterwards conducted on the MS and WPM media supplemented with different growth regulators of IBA, IAA and NAA at 0, 1, 2, 4 and 8 mg/L with 20 replicates. It was shown that the MS medium plus 1 mg/L IBA gave the highest percentage of root induction of 90%, higher root number of 15.65?2.06 roots per shoot and root length of 1.81?0.29 cm. H. siamicum Craib. shoot was induced callus formation of 100% and callus diameter of 2.43?0.13 cm on the WPM medium plus 8 mg/L NAA. The plantlets with both roots and shoots were transferred to various planting materials which were vermiculite, perlite, peat, and the mixture of vermiculite, perlite, and peat at the ratio of 1:1:1 for 30 days in the green house. It was found that H. siamicum Craib. planted in the mixtures of vermiculite, perlite, and peat had 50% survival percentage and average plant height 3.5 cm. |
