A Study of Pathogen-Free Melons for the Most Suitable Age of Tissue Culture Seedlings to Transplant for Commercial Production
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Creator Yuttaya Yuyen, Nattapong Chanchula, Rochana Tangkoolboribun, Khanok-on Amprayn, Peerada Pongtong, and Chatree Konee
Title A Study of Pathogen-Free Melons for the Most Suitable Age of Tissue Culture Seedlings to Transplant for Commercial Production
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Publisher TuEngr Group
Publication Year 2565
Journal Title International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies
Journal Vol. 13
Journal No. 12
Page no. 13A12P: 1-13
Keyword Cucumis melo, Cucumber Mosaic Virus, cucurbits, pathogen-free, seedling age, tissue culture, transplant age
URL Website http://TuEngr.com/Vol13-12.html
Website title ITJEMAST V13(12) 2022 @ TuEngr.com
ISSN 2228-9860
Abstract To determine the most suitable age for in vitro melon seedlings to transplant for growing pathogen-free melons, we designed an RCBD experiment with the two factors being cultivar (Galia and Orange Man) and seedling age at the time of transplant (10, 15, and 20 days compared to 14-day-old control seedlings that were grown conventionally), with 10 specimens per treatment. Measuring mature melon plants at 42 days, we found that for the parameters of plant height, stem circumference, leaf width, and leaf length, the factors of cultivar and seedling age at the time of transplant had a statistically significant combined influence, with plants from 15-day-old Galia seedings exhibiting the greatest average plant height at 232.50ฑ2.44 cm and plants from 15-day-old Orange Man seedlings exhibiting the greatest average stem circumference, leaf width, and leaf length at 1.02ฑ0.05, 20.89ฑ0.33 and 20.56ฑ0.29 cm, respectively. For fruit quality, the melons from 15-day-old Galia seedlings had the greatest average flesh thickness and flesh firmness at 2.56ฑ0.28 cm and 34.86ฑ1.80 Newtons. The age of seedlings at the time of transplant did not have a statistically significant effect on flesh color, rind color, total soluble solids or pH. The most suitable age for transplanting pathogen-free melons raised in tissue culture is 15 days.
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