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Polystyrene Nanoplastic-Induced Toxicity in Neuronal Cells |
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| รหัสดีโอไอ | |
| Creator | Chooi Ling Lim |
| Title | Polystyrene Nanoplastic-Induced Toxicity in Neuronal Cells |
| Contributor | Ming Hui Wong, Rhun Yian Koh, Boon Keat Tan, Yong Qi Leong |
| Publisher | Thai Society of Higher Education Institutes on Environment |
| Publication Year | 2568 |
| Journal Title | EnvironmentAsia |
| Journal Vol. | 18 |
| Journal No. | 1 |
| Page no. | 93-109 |
| Keyword | Polystyrene nanoplastics, SH-SY5Y neuroblastoma cell line, Cytotoxicity, Apoptosis, Reactive oxygen species (ROS) |
| URL Website | http://www.tshe.org/ea/index.html |
| Website title | EnvironmentAsia |
| ISSN | 1906-1714 |
| Abstract | Plastic pollution is a decades-long environmental challenge, contaminating food, water,and even breathable air. Evidence on the permeability of small, fragmented plastic particlescalled nanoplastics through the animal blood brain barrier invoked concerns of potentialneurotoxicity in humans. Thus, this study investigated the mechanism of nanoplastic-inducedtoxicity in a neuronal cell-line. Polystyrene was utilised due to their ubiquity as a plastic pollutantand the second most common plastic type found in human blood. SH-SY5Y neuroblastomacells were exposed to variable concentrations (0.2% to 0.0000128%) and sizes (20 - 100 nm)of polystyrene nanoplastics (PS-NPs). Fluorescence microscopy findings suggest thatPS-NPs were engulfed by SH-SY5Y cells. PS-NP eff ect on cell viability was dependent on theconcentration and size of PS-NPs. Increased PS-NP concentrations (from 0.008%) reducedcell viability, whereby the smallest particle sizes infl icted the strongest cytotoxicity (IC50 of20 nm PS-NPs = 0.0055%) (p < 0.05). Increased intracellular ROS levels were also found inthe treated cells compared to the control. Hoechst 33258 staining and fl ow cytometry suggestedapoptotic induction by 18.18% in 24 hours. In conclusion, PS-NPs showed accumulation andinduced apoptosis in SH-SY5Y cells, suggesting potential neurotoxicity upon human exposureto nanoplastics. |
