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Detection of Foodborne Pathogen Vibrio parahaemolyticus in Milkfish and Nile Tilapia from Selected Wet Markets in Quezon City, Philippines |
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| รหัสดีโอไอ | |
| Creator | Pierangeli G. Vital |
| Title | Detection of Foodborne Pathogen Vibrio parahaemolyticus in Milkfish and Nile Tilapia from Selected Wet Markets in Quezon City, Philippines |
| Contributor | Shaina Lou T. Palar, Nacita B. Lantican |
| Publisher | Thai Society of Higher Education Institutes on Environment |
| Publication Year | 2568 |
| Journal Title | EnvironmentAsia |
| Journal Vol. | 18 |
| Journal No. | 1 |
| Page no. | 126-137 |
| Keyword | Antibiotic resistance, V. parahaemolyticus, Loop-mediated Isothermal Amplification, Polymerase Chain Reaction, tdh gene |
| URL Website | http://www.tshe.org/ea/index.html |
| Website title | EnvironmentAsia |
| ISSN | 1906-1714 |
| Abstract | Vibrio parahaemolyticus is one of the foodborne pathogens causing public concern in bothaquaculture and the medical field. In this study, presence of V. parahaemolyticus was detected in84% (n = 84) of the total fish samples using thiosulfate-citrate-bile salts-sucrose (TCBS) agar,supplemented with 3% salt. Among these, 55.95% (n = 47) were from milkfish, while 44.05%(n = 37) were from tilapia, highlighting the halophilic nature of V. parahaemolyticus. Incidenceof pathogenic V. parahaemolyticus with thermostable-direct hemolysin (tdh) gene was detectedthrough polymerase chain reaction (PCR) (20.24% positivity rate) and loop-mediated isothermalamplification (LAMP) (21.43% positivity rate). In addition, all the confirmed pathogenicV. parahaemolyticus in both amplification methods were subjected to antimicrobial resistanceassay, and were found to be resistant against sulfamethoxazole. Out of the 18 isolates, 28%(n = 5) were resistant to oxytetracycline; whereas 6% (n = 1) were resistant to florfenicol.Overall, 28% (n = 5) V. parahaemolyticus tdh+ isolates were found to be multi-drug resistant.The presence of pathogenic and multi-drug resistant V. parahaemolyticus in food sources is amajor health and farming concern that could affect the sectors of food production, aquaculture,and public health. Thus, the results of this study could serve as the baseline for the developmentof microbiological criteria in aquaculture in the Philippines. |
