Removal of Iron (II) by Burkholderia pseudomallei in Brackish Environment
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Creator 1. Harmin Sulistyaning Titah
2. Rizky Islami Putera
3. Herman Pratikno
4. Atiek Moesriati
Title Removal of Iron (II) by Burkholderia pseudomallei in Brackish Environment
Publisher Thai Society of Higher Education Institutes on Environment
Publication Year 2563
Journal Title EnvironmentAsia
Journal Vol. 13
Journal No. 1
Page no. 75-85
Keyword Bioremediation, Burkholderia pseudomallei, Heavy metal, Kinetics rate, pH, Salinity
URL Website http://www.tshe.org/ea/index.html
Website title EnvironmentAsia
ISSN 1906-1714
Abstract The activity of ship breaking is one of the sources of iron metal pollution in coastal areas and sea water. Iron metal pollution in high concentrations can pose a danger to the organism. Bioremediation of heavy metals by bacteria is the process of altering toxic molecules or metal ions to be reduced levels of toxicity. One of the bacteria isolated and identification of bacteria originating from the ship breaking areas contaminated with iron metal was Burkholderia pseudomallei. This research aimed to determine percentage and kinetic rate of iron (II) removal by Burkholderia pseudomallei. The variables in the research were salinity and pH of medium in laboratory scale. Salinity of medium were used at 15 ppt and 20 ppt. The normal pH and optimum pH at acidic conditions were chosed based on the pH effect test. The initial concentration of iron (II) was 100 mg/L. The iron (II) removal by Burkholderia pseudomallei was performed for 96 hr. The parameters that measured were Optical Density (OD), pH, temperature, iron (II) concentration, and number of bacteria colonies. The results showed that iron (II) removal process optimum at neutral pH. Reactor with pH of 7 showed the percentage of 57.8% and 58.5% at salinity of 15 ppt and 20 ppt, respectively. Salinity affected the kinetics equation of iron (II) removal test. The model of pseudo second order kinetics equation in reactor with neutral pH and salinity of 20 ppt showed well fitted biosorption result (R2 = 0.9668) with constant kinetics rate (k2) of 0.00038 mg/g.hr.
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