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Large scale production of in vitro plantlets of Stevia rebaudiana (Bertoni) using low-cost gelling agents as alternative to commonly used agar |
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| รหัสดีโอไอ | |
| Creator | Nisar Ahmad |
| Title | Large scale production of in vitro plantlets of Stevia rebaudiana (Bertoni) using low-cost gelling agents as alternative to commonly used agar |
| Contributor | Sidra Mukhtar, Naveed Ahmad, Muhammad Sajid, Irfan ullah, Nadia Samad, Muhammad Numan, Zafar Iqbal, Hina Fazal, Mohammad Ali, Hassan Sher |
| Publisher | Khon Kaen University, Thailand |
| Publication Year | 2568 |
| Journal Title | Asia-Pacific Journal of Science and Technology |
| Journal Vol. | 30 |
| Journal No. | 3 |
| Page no. | 3 (11 pages) |
| Keyword | Stevia rebaudiana, Morphogenesis, Gelling agents, Isubgol, Sago, Semolina, Corn starch, BAP |
| URL Website | https://apst.kku.ac.th/ |
| Website title | https://apst.kku.ac.th/large-scale-production-of-in-vitro-plantlets-of-stevia-rebaudiana-bertoni-using-low-cost-gelling-agents-as-alternative-to-commonly-used-agar/ |
| ISSN | 2539-6293 |
| Abstract | Plant cell culture is an advance technology for large scale production of medicinal plants of commercial importance but high cost of the media is one of the major issues for economical growers. One of the most expensive agent of culture media is agar and therefore, the main objective of this study was to select best agar alternative among isubgol, semolina, laundry starch, sago, and corn starch. These alternatives were tested during the morphogenesis of Stevia rebaudiana (Bert.). S. rebaudiana (Bert.) is one the famous sweet herb that produce sweet diterpenes that could be used as sugar alternative for diabetic patients due to its non-toxic nature. Here, the synergistic combinations of isubgol and Agar+isubgol augmented Murashige and Skoog (MS) media supplemented with 2.0 mg/L benzyl aminopurine (BAP) and 0.5 mg/L gibberellic acid (GA3) was found to be effective for shoot induction (100%) and shoot morphogenesis than control (MS media without plant growth regulators). The MS media supplemented with 0.5 mg/L naphthalene acetic acid (NAA) along with corn starch (90 g/L) is the best combination for rapid root initiation, however, corn starch/sago alone or the synergistic combination of with agar or sago significantly improved root organogenesis (100%) in medicinally important Stevia rebaudiana (Bert.). However, sago encourage mean shoot and root length and reduced 90% cost than agar. Semolina was the cheapest agar substitute by reducing the cost up to 97.75% and could be scale up for higher production of plantlets of commercial importance. |
