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Heavy Metal Tolerance and Removal Capacity of Trichoderma species Isolated from Mine Tailings in Itogon, Benguet |
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| รหัสดีโอไอ | |
| Creator | Myra Tansengco, Judith Tejano, Fe Coronado, Carmel Gacho and Joven BarceloJoven Barcelo |
| Title | Heavy Metal Tolerance and Removal Capacity of Trichoderma species Isolated from Mine Tailings in Itogon, Benguet |
| Publisher | Faculty of Environment and Resource Studies, Mahidol University |
| Publication Year | 2561 |
| Journal Title | Environment and Natural Resources Journal |
| Journal Vol. | 16 |
| Journal No. | 1 |
| Page no. | 39 to 57 |
| Keyword | Heavy metal/ Fungi/ Trichoderma/ Itogon/ Benguet/ Mining/ Mine tailings |
| ISSN | 1686-5456 |
| Abstract | Waste from mining industries contains various heavy metals that can pollute the environment. Bioremediation using efficient fungi can help in eliminating these heavy metal contaminants. This study focused on the isolation, identification, and characterization of heavy metal-resistant fungi from mine tailings in Itogon, Benguet. Isolation of fungi was done by serial dilution and spread plate techniques on potato dextrose agar (PDA) with an individual heavy metal, i.e. chromium (Cr), copper (Cu), lead (Pb), zinc (Zn), and nickel (Ni). Of the 29 fungal isolates, four species were selected and molecularly identified as Trichoderma virens, T. harzianum, T. saturnisporum, and T. gamsii. Growth tolerance on PDA with increasing concentrations (200-1000 ppm) of an individual heavy metal indicated the following trend: T. virens > T. harzianum > T. gamsii > T. saturnisporum. Growth test indicates that all Trichoderma isolates can tolerate high levels of Cr and Pb, however tolerance to Cu, Zn, and Ni was species specific. Shakeflask culture using T. virens showed high lead removal (91-96%) over broad pH range while and at neutral pH, T. virens had 70% and 63% reductions for Cu and Cr, respectively. Results of this study highlights the potential of Trichoderma isolates for biological wastewater treatment in mining industries. |
