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Rice extracts (KDML105 and PL2 cultivars) modulate lipidaccumulation, oxidative status, and inflammationin oxidative stress-induced 3T3-L1 adipocytes |
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| รหัสดีโอไอ | |
| Creator | 1. Wajathip Bulanawichit 2. Sakara Tunsophon 3. Tantip Boonsong |
| Title | Rice extracts (KDML105 and PL2 cultivars) modulate lipidaccumulation, oxidative status, and inflammationin oxidative stress-induced 3T3-L1 adipocytes |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2565 |
| Journal Title | Songklanakarin Journal of Science an Technology (SJST) |
| Journal Vol. | 44 |
| Journal No. | 2 |
| Page no. | 576-583 |
| Keyword | rice extracts, lipid accumulation, oxidative stress, obesity |
| URL Website | https://rdo.psu.ac.th/sjst/index.php |
| ISSN | 0125-3395 |
| Abstract | High reactive oxygen species (ROS) level accumulations are coupled with alterations of pro- and anti-inflammatorycytokines, and antioxidant system abnormalities, and they contribute in adipocytes with developing obesity. This study aimed toinvestigate the effects of rice extracts from KDML105 and PL2 cultivars on lipid accumulation, oxidative status, andinflammatory gene and protein expression in 3T3-L1 adipocytes. Cells were treated with rice extracts (GBR, BR, and WR). GBRand BR extracts significantly decreased intracellular ROS levels and lipid accumulation by DCFH-DA assay and oil-red Ostaining. Further, qPCR also showed a decrease in the mRNA expression of TNF-๏ก and IL-6. Conversely, the rice extractsincreased the expressions of antioxidants (Nrf2, HO-1, GPx4, CAT), and the adiponectin gene, and the expression of theadiponectin SOD2 and GPx4 proteins. These results demonstrated an inhibitory effect of both the GBR and BR extracts on lipidaccumulation and regulated the expression of oxidative stress, inflammatory genes, and the proteins in H2O2-induced 3T3-L1adipocytes. Due to anti-adipogenesis and the oxidative status of GABA and ๏ง-oryzanol, the germinated rice extracts can beserved as a beneficial supplement for the prevention and/or treatment of obesity, and oxidative-related diseases. |
