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Chimeric MrNV-GE11-VLPs serve as a nano-containerto deliver Doxorubicin into cancer cells |
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| รหัสดีโอไอ | |
| Creator | 1. Khwanthana Grataitong 2. Charoonroj Chotwiwatthanakun 3. Orawan Thongsum 4. Krittalak Chakrabandhu 5. Wattana Weerachatyanukul |
| Title | Chimeric MrNV-GE11-VLPs serve as a nano-containerto deliver Doxorubicin into cancer cells |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2565 |
| Journal Title | Songklanakarin Journal of Science an Technology (SJST) |
| Journal Vol. | 44 |
| Journal No. | 6 |
| Page no. | 1517-1523 |
| Keyword | MrNV, VLP, colorectal cancer, EGFR, doxorubicin |
| URL Website | https://sjst.psu.ac.th/ |
| ISSN | 0125-3395 |
| Abstract | We have reported that virus-like particle from shrimp virus, MrNV-VLP, effectively encapsulates and delivers plasmidDNA and dsRNA into Sf9 insect cells and shrimp tissues. Additionally, modifying VLP with GE-11 peptide extension on thesurface (so called, E-MrNV-GE11-VLP) allows them to interact specifically with the EGFR-positive SW480 cancer cells. Thiswork extrapolated the use of E-MrNV-GE11-VLP to encapsulate and deliver doxorubicin (DOX) towards SW480 cells. Theresults showed that DOX was passively loaded into VLPs in a molar ratio of >200 DOX/VLP equivalent to a loading efficiencyof 3%. Specific targeting of E-MrNV-GE11-VLP + DOX and its anti-cancer effect towards SW480 was more pronounced thanthat of N-MrNV-VLP + DOX, suggesting an interaction and internalization of E-MrNV-GE11-VLP through surface EGFR. Thisclaim was also supported by a lower DOX delivery into MCF7 than SW480 cells. Finally, the cell cytotoxicity assay showed thatE-MrNV-GE11-VLP + DOX significantly decreased cell viability in SW480 cells more than that by N-MrNV-VLP + DOX(P<0.05), while its cytotoxicity effect on MFC7 cells was much lower than on SW480 cells. This study provides insights intohow to develop target-specific drug delivery for carrying therapeutic agents towards specific tumor cells. |
