|
Caco-2 models for xenobiotic metabolism and transportation |
|---|---|
| รหัสดีโอไอ | |
| Creator | 1. Nathaphon Kuncharoenwirat 2. Waranya Chatuphonprasert 3. Kanokwan Jarukamjorn |
| Title | Caco-2 models for xenobiotic metabolism and transportation |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2565 |
| Journal Title | Songklanakarin Journal of Science an Technology (SJST) |
| Journal Vol. | 44 |
| Journal No. | 5 |
| Page no. | 1381-1389 |
| Keyword | colon carcinoma cell, Caco-2, cytochrome P450, transporter, intestinal model |
| URL Website | https://sjst.psu.ac.th/ |
| ISSN | 0125-3395 |
| Abstract | The intestine plays an important role in the absorption, metabolism, and transportation of xenobiotics including drugsand foods. Therefore, an in vitro intestinal model for predicting bioavailability of xenobiotics is important for the drug and foodindustries. Caco-2 is a human colon carcinoma cell line widely employed as a model to represent xenobiotic absorption,transportation, and metabolism in the human intestinal tract. However, Caco-2 has limitations as an intestinal model due to thelow expression of metabolizing enzymes and the long time period required for cell differentiation. Hence, several modified Caco2 models have been developed to overcome these limitations and to establish Caco-2 models that correlate well with humanintestine. These include xenobiotic-induced cytochrome P450 and transporter expression models, nuclear receptor gene-modifiedCaco-2 cells, and a novel Caco-2 culturing method that mimics the physiological characteristics of the human intestine andaccelerates the growth rate of Caco-2. |
