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The SARS-CoV-2 infections in Thailand: Analysis of spike mutations complemented by protein structure insights |
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| รหัสดีโอไอ | |
| Creator | 1. Sirawit Ittisoponpisan 2. Shalip Yahangkiakan 3. Michael J E Sternberg 4. Alessia David |
| Title | The SARS-CoV-2 infections in Thailand: Analysis of spike mutations complemented by protein structure insights |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2565 |
| Journal Title | Songklanakarin Journal of Science an Technology (SJST) |
| Journal Vol. | 44 |
| Journal No. | 5 |
| Page no. | 1201-1208 |
| Keyword | SARS-CoV-2, mutation analysis, Angiotensin-converting enzyme 2, spike protein, Thailand |
| URL Website | https://sjst.psu.ac.th/ |
| ISSN | 0125-3395 |
| Abstract | Thailand was the first country outside China to officially report COVID-19 cases. With a large number of SARS-CoV2 sequences collected from patients, the effects of many genetic variations, especially those unique to Thai strains, are yet to beelucidated. In this study, we analyzed 439,197 sequences of the SARS-CoV-2 spike protein collected from NCBI and GISAIDdatabases. 595 sequences were from Thailand and contained 52 amino acid mutations, of which 6 had not been observed outsideThailand (p.T51N, p.P57T, p.I68R, p.S205T, p.K278T, p.G832C). These mutations were not predicted to be of concern. Wedemonstrate that p.D614G became the prevalent strain during the second outbreak, and the most common spike mutationsdetected in Thailand (p.A829T, p.S459F and p.S939F) do not appear to cause any major structural change to the spike trimer orthe spike-ACE2 interaction. Among the spike mutations identified in Thailand was p.N501T. This mutation was not predicted toincrease SARS-CoV-2 binding, in contrast to the spike mutation of interest p.N501Y. In conclusion, Thailand-specific mutationsare unlikely to increase the fitness of SARS-CoV-2. The insights obtained from this study could aid in prioritizing SARS-CoV-2variants and in strain surveillance. |
