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Cryoprotectants for cryopreservation of spermatozoa of tropical oysterMagallana bilineata (Rรถding, 1798) |
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| รหัสดีโอไอ | |
| Creator | 1. Poon Wing Yee 2. Noorain Ilias 3. Nithiyaa Nilamani 4. Teh Chiew Peng 5. Aileen Tan Shau Hwai |
| Title | Cryoprotectants for cryopreservation of spermatozoa of tropical oysterMagallana bilineata (Rรถding, 1798) |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2565 |
| Journal Title | Songklanakarin Journal of Science an Technology (SJST) |
| Journal Vol. | 44 |
| Journal No. | 1 |
| Page no. | 91-96 |
| Keyword | cryopreservation, spermatozoa, fertilization rate, tropical oysters |
| URL Website | https://rdo.psu.ac.th/sjst/index.php |
| ISSN | 0125-3395 |
| Abstract | Cryopreservation is a freezing method specific for cells such as gametes and tissues in a sub-zero temperature. The roleof cryoprotectant (cryoprotective agent) is essential to reduce the cryoinjury and retain the original biological function of gametesduring cryopreservation. This study looked into the effectiveness of four types of cryoprotectants (dimethyl sulfoxide, sucrose,egg yolk and honey) at four concentrations (5%, 10%, 15%, and 20%) on the spermatozoa of tropical oyster, Magallanabilineata. Sperm exposed to DMSO 10% underwent freezing has recorded the highest fertilization rate, which was significantlyhigher than other treatments but showed no significant differences with the control (fresh sperm). The usage of sucrose is lesseffective compared to DMSO, but showed no significant differences with egg yolk. This study is important to determine the bestcryoprotectant to be used for spermatozoa cryopreservation of M. bilineata, to provide a solution to oyster hatchery operators tosecure all year-round spawning using cryopreserved sperms. |
