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Transcriptome profile and pathway analysis of starchand sucrose metabolism in Euglena gracilis |
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| รหัสดีโอไอ | |
| Creator | 1. Wenhui Zhang 2. Wen Jye Mok 3. Jinwei Gao 4. Yeong Yik Sung 5. Wenli Zhou |
| Title | Transcriptome profile and pathway analysis of starchand sucrose metabolism in Euglena gracilis |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2565 |
| Journal Title | Songklanakarin Journal of Science an Technology (SJST) |
| Journal Vol. | 44 |
| Journal No. | 4 |
| Page no. | 1048-1056 |
| Keyword | Euglena gracilis, high-throughput sequencing, transcriptome, starch and sucrose metabolism |
| URL Website | https://rdo.psu.ac.th/sjst/index.php |
| ISSN | 0125-3395 |
| Abstract | Euglena gracilis, a single cell flagellate eukaryote, produces secondary metabolites known as paramylon, acarbohydrate similar to starch with beneficial health properties. In this study, we sequenced the transcriptome and identified thepathways and key enzymes involved in the starch and sucrose metabolism of E. gracilis. A total of 120,086 unigenes wereassembled with the Trinity software, of which 48,031 were annotated. The genes involved in the starch and sucrose metabolismof E. gracilis were further identified, leading to the construction of a preliminary pathway map of starch metabolism for thissingle cell flagellate eukaryote species. Both ฮฒ-amy and Spase2 were identified to play major roles in starch catabolism, whereasglucose-1-phosphate adenylyltransferase functions in starch anabolism. It is anticipated that the starch content of E. gracilis caneither be augmented by inhibiting the starch breakdown with ฮฒ-amy or Spase2 gene interference or by elevating the level ofAGPase activity of Glucose-1-phosphate transferase to promote starch anabolism. Whatever the outcomes, results from this studyprovided a fundamental understanding on how E. gracilis regulates its starch metabolic pathways, and the impact of geneticmodification on starch synthesis. |
