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Adverse effects of cypermethrin on golden apple snails(Pomacea canaliculata) and their eggs, and application ofAcetylcholinesterase (AChE) as biomarker |
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| รหัสดีโอไอ | |
| Creator | 1. Phochit Nanthanawat 2. Samnao Saowakoon 3. Witchuda Prasatkaew 4. Amnuay Wattanakornsiri |
| Title | Adverse effects of cypermethrin on golden apple snails(Pomacea canaliculata) and their eggs, and application ofAcetylcholinesterase (AChE) as biomarker |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2565 |
| Journal Title | Songklanakarin Journal of Science an Technology (SJST) |
| Journal Vol. | 44 |
| Journal No. | 4 |
| Page no. | 1040-1047 |
| Keyword | ELISA technique, mortality rate, toxicity testing, western blot |
| URL Website | https://rdo.psu.ac.th/sjst/index.php |
| ISSN | 0125-3395 |
| Abstract | This study aimed to evaluate the effects of cypermethrin on hatching rate of golden apple snail eggs, mortality rate ofthe golden apple snails, and acetylcholinesterase (AChE) expression applied as a bio-indicator. The results showed thatcypermethrin concentration did not affect hatching rate or development of the eggs and larvae in comparison with the control.The mortality rate depended on the exposure concentration. Median lethal concentration (LC50) (95% confidence) at 96 h wasapproximately 8.99 (8.93-9.06). The concentration of cypermethrin had an effect on AChE expression in both the snails and theireggs. The molecular weight of AChE found was 71 kDa, as studied by SDS-PAGE and Western blot techniques. The ELISAtechnique revealed that AChE contents in both the snails and their eggs were significantly different from the control (p <0.05).Based on our results, AChE could be applied to assess cypermethrin exposure in the snails and their eggs, in order to plancontamination management of such pesticides in the snails and reduce the risks to consumers. |
