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Potential of Chlorella sp. exopolysaccharide as adjuvant forMannheimia haemolytica A2 vaccine in rat model |
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| รหัสดีโอไอ | |
| Creator | 1. Insyirah-husna Kamaradin 2. Suvik Assaw 3. Hassan I. Sheikh 4. Mohd Effendy Abd Wahid |
| Title | Potential of Chlorella sp. exopolysaccharide as adjuvant forMannheimia haemolytica A2 vaccine in rat model |
| Publisher | Research and Development Office,Prince of Songkla University |
| Publication Year | 2564 |
| Journal Title | Songklanakarin Journal of Science and Technology (SJST) |
| Journal Vol. | 43 |
| Journal No. | 3 |
| Page no. | 752-760 |
| Keyword | exopolysaccharide, Mannheimia haemolytica A2, adjuvant, immunoglobulins, intranasal |
| URL Website | https://rdo.psu.ac.th/sjstweb/ |
| ISSN | 0125-3395 |
| Abstract | The potential of exopolysaccharides (EPS) extracted from Chlorella sp. as adjuvant for formalin-killed whole organismof Mannheimia haemolytica A2 (MhA2) vaccine was investigated. Chlorella sp. was cultured in F/2 media and EPS wasextracted and characterized using GPC, FTIR and SEM, while its cytotoxicity was tested on RAW 264.7. Sprague Dawley ratswere inoculated intranasally with either phosphate-buffered saline (PBS) (Group A), EPS only (Group B), formalin killed wholeorganism of MhA2 vaccine seed only (Group C) or EPS-MhA2 adjuvanted vaccine (Group D). Serum levels of IgM, IgG, andIgA titers were collected for nine weeks. About 4.8 mg of EPS was obtained in the form of white powder with molecular weightof 2398 Da. The microstructure of EPS was compact and exhibited flakes like structural units and was not toxic to RAW 264.7.EPS-MhA2 group produced significantly higher (p<0.05) IgM, IgG and IgA responses compared to other groups. |
