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Phytoestrogen constituents and estrogenic activity ofPueraria candollei var. mirifica callus and its extract preparation forremoving cytotoxic constituents |
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| รหัสดีโอไอ | |
| Creator | 1. Witsarut Kraithong 2. Wipawee Juengsanguanpornsuk 3. Supaluk Krittanai 4. Gorawit Yusakul 5. Waraporn Putalun |
| Title | Phytoestrogen constituents and estrogenic activity ofPueraria candollei var. mirifica callus and its extract preparation forremoving cytotoxic constituents |
| Publisher | Research and Development Office,Prince of Songkla University |
| Publication Year | 2564 |
| Journal Title | Songklanakarin Journal of Science and Technology (SJST) |
| Journal Vol. | 43 |
| Journal No. | 3 |
| Page no. | 703-710 |
| Keyword | Pueraria candollei var. Mirifica, isoflavonoids, chromenes, estrogenic activity |
| URL Website | https://rdo.psu.ac.th/sjstweb/ |
| ISSN | 0125-3395 |
| Abstract | In vitro cultures of Pueraria candollei var. mirifica (PC) are a promising source of phytoestrogens, however, theestrogenic activity of PC callus has not been evaluated. In this study, the phytochemicals of PC callus were analyzed andcompared with the estrogenic activities on MCF-7 cell proliferation. The ethanolic extract of PC callus contained isoflavonoidsand deoxymiroestrol. The estrogenic activity of the extract (10 ยตg/mL) was equivalent to 1ร10-10 M estradiol. When the crudeextract was reconstituted with 10% (v/v) ethanol in water, most of the isoflavonoid and deoxymiroestrol contents weresolubilized. The soluble fraction exhibited the estrogenic effect in a concentration-dependent manner, but treatment with theinsoluble fraction at a high concentration (100 ยตg/mL) resulted in cell growth suppression. Overall, the crude ethanolic extractand 10 %(v/v) ethanol-soluble crude extract of PC callus were good sources of phytoestrogens. The reconstitution could removetoxic constituents, but the estrogenic activity was retained. |
