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Effect of n-butanol fraction of Detarium microcarpum stem barkon some liver function and oxidative stress parametersin carbon tetrachloride induced hepatic injury in Wistar rats |
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| รหัสดีโอไอ | |
| Creator | 1. Theresa Yebo Gara 2. Danladi Amodu Ameh 3. Kola Mattew Anigo 4. Augustine Innalegwu Daniel |
| Title | Effect of n-butanol fraction of Detarium microcarpum stem barkon some liver function and oxidative stress parametersin carbon tetrachloride induced hepatic injury in Wistar rats |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2564 |
| Journal Title | Songklanakarin Journal of Science and Technology (SJST) |
| Journal Vol. | 43 |
| Journal No. | 1 |
| Page no. | 312-317 |
| Keyword | Detarium microcarpum, hepatotoxicity, carbon-tetrachloride, liver function, oxidative stress |
| URL Website | https://rdo.psu.ac.th/sjstweb/index.php |
| ISSN | 0125-3395 |
| Abstract | Hepatic damage is a serious health challenge that alters normal metabolic processes in the body. Total flavonoid, phenoliccontent and lethal dose (LD50) of n-butanol fraction of Detarium microcarpum stem bark were evaluated using standard procedures.The effect of n-butanol fraction of Detarium microcarpum stem bark on the relative liver weight, some liver functions (Alanineaminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and bilirubin (Bil)) and oxidative stress(malondialdehyde MDA, superoxide dismutase SOD, and catalase CAT) parameters in carbon-tetrachloride (CCl4) induced hepaticinjury in Wistar rats were investigated. Experimental animals were divided into 6 groups, to induce hepatic injury. CCl4 in oliveoil (1:1) was introduced by intraperitoneal injection once a week for 4 weeks and graded doses (100, 150 and 200) mg/ml of nbutanol fraction of Detarium microcarpum stem bark were orally administered, daily for 28 days. The quantity of total flavonoidsand phenols in n-butanol fraction of Detarium microcarpum stem bark were 234.42 ? 0.71 and 2.97 ? 0.31 (mg/g) respectively.The LD50 of n-butanol fraction of Detarium microcarpum stem bark was >5000 mg/kg body weight of rats. The relative liverweight of all the treated groups decreased significantly (p<0.05) compared to the CCl4 only treatment (negative control). ALT,AST, ALP, Bil and MDA significantly decreased while SOD and CAT increased significantly compared with the negative control.These results suggest that Detarium microcarpum stem bark contains phytochemicals with antioxidant properties and holds promisein ameliorating liver injuries. |
