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Non-mutagenic and genotoxic effects of water extract ofPiper sarmentosum using Ames and micronucleus assay |
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| รหัสดีโอไอ | |
| Creator | 1. Achiraya Tammasakchai 2. Penchom Peungvicha 3. Rungravi Temsiririrkkul 4. Pongpun Siripong 5. Pranom Puchadapirom |
| Title | Non-mutagenic and genotoxic effects of water extract ofPiper sarmentosum using Ames and micronucleus assay |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2564 |
| Journal Title | Songklanakarin Journal of Science and Technology (SJST) |
| Journal Vol. | 43 |
| Journal No. | 1 |
| Page no. | 203-209 |
| Keyword | Piper sarmentosum, mutagenicity, genotoxicity, Ames assay, micronucleus assay |
| URL Website | https://rdo.psu.ac.th/sjstweb/index.php |
| ISSN | 0125-3395 |
| Abstract | P. sarmentosum possesses a variety of biological activities and has long been used as a food ingredient and traditionalmedicine to treat many diseases. In this study, its mutagenic and genotoxic activities on S. typhimurium TA98 and TA100 andfibroblast (V79) cells were examined. Mediated by S9 activation, the water extract of P. sarmentosum at a high dose caused thenumber of revertant colonies to increase in the tester strains TA98 (20 mg/plate) and TA100 (40 mg/plate), but there was nomutagenic effect in the presence of S9 in mixed condition. The extract (1 - 7.5 mg/ml) showed no significant genotoxic effectbased on the frequency of micronucleus formation both with and without S9 in mixed conditions. In conclusion, the water extractof P. sarmentosum has neither direct nor indirect genotoxic effects and has no mutagenic effects indirectly. Therefore, the resultsindicate that P. sarmentosum is safe for use as a food supplement. |
