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Development of in vitro culture system for proliferation of protocormlike bodies of Oncidium Golden Anniversary orchid |
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รหัสดีโอไอ | |
Creator | 1. Suhana Zakaria 2. Jessica Jeyanthi James Antony 3. Chee Keong Chin 4. Rahmad Zakaria 5. Sreeramanan Subramaniam |
Title | Development of in vitro culture system for proliferation of protocormlike bodies of Oncidium Golden Anniversary orchid |
Publisher | Research and Development Office, Prince of Songkla University |
Publication Year | 2564 |
Journal Title | Songklanakarin Journal of Science an Technology (SJST) |
Journal Vol. | 43 |
Journal No. | 6 |
Page no. | 1542-1549 |
Keyword | Oncidium Golden Anniversary, protocorm-like bodies, growth index, thin cell layer |
URL Website | https://rdo.psu.ac.th/sjst/index.php |
ISSN | 0125-3395 |
Abstract | Oncidium orchid has high demand and economic value especially in floriculture industry. This study aimed to optimizea suitable medium for rapid proliferation of protocorm-like bodies (PLBs) of Oncidium Golden Anniversary orchid. Results fromthis study confirmed that ยฝ MS semi-solid medium displayed the highest growth index and total numbers of PLBs. Inclusion of20g/L sucrose and 1mg/L BAP produced the highest PLB proliferation rate, while adding charcoal gave no significant effect.Half-moon PLB size contributes to the highest growth rate on using a thin cell layer (TCL) system. Hence, the best medium forOncidium Golden Anniversary PLB proliferation was ยฝ MS semi-solid media supplemented with 20 g/L sucrose and 1 mg/LBAP. |