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Separation of diethylpyrocarbonate-modified histidine-containingpeptides using strong cation exchange chromatography |
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| รหัสดีโอไอ | |
| Creator | 1. Pongsakorn Thawonpan 2. Supinya Thanapongpicha 3. Watthanachai Jumpathong 4. Aung Win Tun 5. Churat Weeraphan 6. Worrawit Wanitsuwan 7. Hansuk Buncherd |
| Title | Separation of diethylpyrocarbonate-modified histidine-containingpeptides using strong cation exchange chromatography |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2564 |
| Journal Title | Songklanakarin Journal of Science an Technology (SJST) |
| Journal Vol. | 43 |
| Journal No. | 5 |
| Page no. | 1443-1448 |
| Keyword | histidine modification, diethylpyrocarbonate, strong cation exchange chromatography, imidazole ring, carbethoxyimidazol |
| URL Website | https://rdo.psu.ac.th/sjst/index.php |
| ISSN | 0125-3395 |
| Abstract | This work focuses on the separation of diethylpyrocarbonate-modified histidine-containing peptides by strong cationexchange (SCX) chromatography prior to mass spectrometric analysis. The decrease in positive charge of diethylpyrocarbonatetreated imidazole in histidine, masked by the incorporation of the carbethoxyl group, provides us with an opportunity to use SCXfor separating diethylpyrocarbonate-modified histidine-containing peptides from the unmodified ones. In this report, thediethylpyrocarbonate-modified histidine-containing peptide is eluted faster than the untreated peptide. Furthermore, thecarbethoxy group can be effectively removed to generate the unmodified imidazole in the histidine residue by neutralhydroxylamine. |
