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Proteomic analyses in fast and slow-cleaving bovine embryos a searchfor potential markers of healthy pre-implantation embryos |
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| รหัสดีโอไอ | |
| Creator | 1. Preeyalak Konongbua 2. Atthaboon Watthammawut 3. Sittiruk Roytrakul 4. Monsicha Somrit 5. Somluk Asuvapongpatana 6. Wattana Weerachatyanukul |
| Title | Proteomic analyses in fast and slow-cleaving bovine embryos a searchfor potential markers of healthy pre-implantation embryos |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2564 |
| Journal Title | Songklanakarin Journal of Science an Technology (SJST) |
| Journal Vol. | 43 |
| Journal No. | 5 |
| Page no. | 1292-1298 |
| Keyword | proteomics, oocyte, bovine, embryo, blastocyst |
| URL Website | https://rdo.psu.ac.th/sjst/index.php |
| ISSN | 0125-3395 |
| Abstract | Selection of embryos with potentially successful development is paramount to ensuring their viability and growth.While morphological assessment has been the mainstay of embryo selection, the identification of proteins that are expressed inthe more reproductively successful fast-cleaving embryos as opposed to slow-cleaving ones may serve to reduce visualassessment of subjectivity in bovine. Through the use of proteomic mass spectrometric techniques, we found that 2387 proteinswere commonly identified in both fast and slow-cleaving embryos, while 464 proteins were differentially identified. Slowcleaving embryos showed a higher percentage of identified proteins involved in apoptotic processes, DNA repair and stressresponses, while fast-cleaving bovine embryos showed a higher percentage of proteins identified that are involved in cellmetabolism, gene transcription, and signal transduction. These differential protein expressions identified in the fast- and slowcleaving embryos may serve as alternative potential markers of healthy pre-implantation embryos and improve overall bovinereproductive rate. |
