|
Factors influencing cell differentiation and expressions of cytochromeP450s and transporters in Caco-2 cells |
|---|---|
| รหัสดีโอไอ | |
| Creator | 1. Nathaphon Kuncharoenwirat 2. Waranya Chatuphonprasert 3. Kanokwan Jarukamjorn |
| Title | Factors influencing cell differentiation and expressions of cytochromeP450s and transporters in Caco-2 cells |
| Publisher | Research and Development Office,Prince of Songkla University |
| Publication Year | 2564 |
| Journal Title | Songklanakarin Journal of Science and Technology (SJST) |
| Journal Vol. | 43 |
| Journal No. | 3 |
| Page no. | 802-809 |
| Keyword | cytochrome P450, transporter, Caco-2, differentiation, intestinal enterocyte |
| URL Website | https://rdo.psu.ac.th/sjstweb/ |
| ISSN | 0125-3395 |
| Abstract | Absorption, transportation, and first-pass metabolism of xenobiotics, drugs and nutrients taken orally occurs in theintestine, which is a major organ expressing cytochrome P450 enzymes (CYP) and transporters. Caco-2 is a human coloncarcinoma cell line widely employed as a model for xenobiotic absorption, transportation, and metabolism in the intestinal tractas it can spontaneously differentiate to become small intestinal enterocytes. Culturing factors influence differentiation of Caco-2as well as expression of CYPs and drug transporters. Culturing period, medium composition, type of filter membrane inserts, andthe seeding density, account for the major factors affecting cell differentiation and the expression of CYP and transporter.Therefore, determining the optimal conditions for Caco-2 culturing is of strong concern depending on the aim and experimentaldesign of the study. |
