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Probiotic characterization and in vitro cholesterol lowering effects oflactic acid bacteria isolated from healthy Thai infants |
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| รหัสดีโอไอ | |
| Creator | 1. Nutcha Sirichotinun 2. Ulisa Pachekrepapol 3. Kwannan Nantavisai 4. Malai Taweechotipatr 5. Sirinun Nilwarangkoon |
| Title | Probiotic characterization and in vitro cholesterol lowering effects oflactic acid bacteria isolated from healthy Thai infants |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2563 |
| Journal Title | Songklanakarin Journal of Science and Technology |
| Journal Vol. | 42 |
| Journal No. | 3 |
| Page no. | 671-677 |
| Keyword | bile salt hydrolase, probiotics, lactic acid bacteria, cholesterol, infant feces |
| URL Website | https://rdo.psu.ac.th/sjstweb/index.php |
| ISSN | 0125-3395 |
| Abstract | Hypercholesterolemia is one of the major health problems affecting people worldwide. Some probiotic lactic acidbacteria possess cholesterol lowering ability. One-hundred lactic acid bacteria isolated from the feces of healthy Thai infantswere screened for bile salt hydrolase activity and cholesterol assimilation ability. Seven isolates expressed strong bile salthydrolase activity and 7 isolates exhibited cholesterol assimilation properties. In total, 11 probiotic candidates were characterizedfor their general probiotic properties including acid and bile tolerance, adherence to Caco-2 cells and genotypic identificationusing 16S rRNA gene sequencing. Eight isolates were identified as Enterococcus faecalis and others were E. faecium, E. durans,and Lactococcus garvieae. All selected isolates survived after incubation at pH 3 and 4, and in 0.3% and 0.8% bile. The strain E.faecium MSMC 25-2 exhibited the highest level of adhesion to Caco-2 cells at 8.8% compared to L. rhamnosus GG. |
