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In vitro biological activities of Thunbergia laurifolia stemand leaf with reference to rosmarinic acid |
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| รหัสดีโอไอ | |
| Creator | 1. Parichart Hongsing 2. Chanida Palanuvej 3. Nijsiri Ruangrungsi |
| Title | In vitro biological activities of Thunbergia laurifolia stemand leaf with reference to rosmarinic acid |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2563 |
| Journal Title | Songklanakarin Journal of Science and Technology |
| Journal Vol. | 42 |
| Journal No. | 3 |
| Page no. | 615-620 |
| Keyword | Thunbergia laurifolia, rosmarinic acid, reactive oxygen species, antioxidant activity |
| URL Website | https://rdo.psu.ac.th/sjstweb/index.php |
| ISSN | 0125-3395 |
| Abstract | Thunbergia laurifolia Lindl. (Thunbergiaceae family) was shown to be a natural antioxidant source to cope withoxidative stress. By DPPH and FRAP antioxidant assays, this study found that rosmarinic acid exhibited the highest antioxidantactivity followed by leaf and stem ethanolic extracts of T. laurifolia. By intracellular anti-oxidation assay against H2O2-inducedROS in EA.hy926 endothelial cells using 2?,7?-dichlorodihydrofluorescein diacetate as a probe, all tested samples exhibitedmarginal protection, i.e. T. laurifolia leaf extract (77.31?0.66%), rosmarinic acid (88.10?6.105%), and T. laurifolia stem extract(91.30?0.86%), compared to the control (100%). Cytotoxicity against five cancer and two normal cell lines were evaluated byMTT cell viability assay. Rosmarinic acid and the leaf extract inhibited cell viability selectively in each cell line, whereas thestem extract revealed no cytotoxicity (IC50?100 ?g/mL) in any of the cell lines |
