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Quality evaluation using TLC methods with reference to brazilin contentof Caesalpinia sappan heartwoods in Thailand |
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| รหัสดีโอไอ | |
| Creator | 1. Suwipa Intakhiao 2. Chanida Palanuvej 3. Nijsiri Ruangrungsi |
| Title | Quality evaluation using TLC methods with reference to brazilin contentof Caesalpinia sappan heartwoods in Thailand |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2563 |
| Journal Title | Songklanakarin Journal of Science and Technology |
| Journal Vol. | 42 |
| Journal No. | 1 |
| Page no. | 196-202 |
| Keyword | Caesalpinia sappan heartwood, brazilin, pharmacognostic specification, TLC-image analysis, TLC-densitometry |
| URL Website | https://rdo.psu.ac.th/sjstweb/index.php |
| ISSN | 0125-3395 |
| Abstract | Caesalpinia sappan L. is a medicinal plant generally used to promote blood circulation and as an anti-thirst remedy.Fifteen samples were collected from different locations in Thailand. The aim was to identify parameters including the anatomicaland histological characters as well as the thin layer chromatography (TLC) fingerprint. The quality parameter results of total ash,acid insoluble ash, ethanol and water soluble extractive values, loss on drying, and water content were 0.87?0.14, 0.44?0.14,2.94?0.57, 3.77?0.64, 8.50?0.37, and 8.52?0.43% by weight, respectively. TLC-densitometry and TLC-image analysis wereperformed to detect brazilin using silica gel 60 GF254 as the stationary phase and chloroform, ethyl acetate, and formic acid(10:8:2) as the mobile phase. The results of brazilin from the TLC-densitometry and TLC-image analysis were 1.259?0.285g/100 g and 1.256?0.266 g/100 g of dried heartwood, respectively. The result showed that both techniques were not statisticallysignificantly different. |
