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Aptamer-gelatin composite material for prolonging PDGF-BB release |
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| รหัสดีโอไอ | |
| Creator | 1. Boonchoy Soontornworajit 2. Karnjana Kerdsiri 3. Thawatchai Tungkavet 4. Anuvat Sirivat |
| Title | Aptamer-gelatin composite material for prolonging PDGF-BB release |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2563 |
| Journal Title | Songklanakarin Journal of Science and Technology |
| Journal Vol. | 42 |
| Journal No. | 1 |
| Page no. | 180-187 |
| Keyword | gelatin, aptamer, composite, PDGF-BB, protein release |
| URL Website | https://rdo.psu.ac.th/sjstweb/index.php |
| ISSN | 0125-3395 |
| Abstract | Platelet-derived growth factor-BB (PDGF-BB) is a promising molecule with a range of medical applications. However,the very rapid delivery of this growth factor may render treatment ineffective. A delivery system that is able to prolong the releaserate is therefore required. In this study, a composite was fabricated from gelatin type IV and an aptamer. The aptamer worked as abinding ligand for PDGF-BB and was shown to slow the release rate. An inverted-tube test showed that the phase transitiontemperature of the gelatin increased in line with the gelatin concentration and in the presence of the coupling reagents N,N'-dicyclohexylcarbodiimide and N-hydroxysuccinimide. Binding functionality of the aptamer was confirmed by surface plasmonresonance spectrometry. Rheological measurements indicated that the incorporation had no effect on the mechanical properties ofthe composite. The degradation of the composite and the kinetic release of the target proteins were investigated. The gelatin wasshown to gradually dissolve in the release medium. The rate of PDGF-BB release from the aptamer-gelatin composite wassignificantly slower than from the native gelatin. This novel composite has potential applications in a range of PDGF-BB-relatedtreatments. |
