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Species identification of forensically important fly larvaeby integrating morphological characteristics and protein profiles |
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| รหัสดีโอไอ | |
| Creator | 1. Pluemkamon Phuwanatsarunya 2. Nuttanan Hongsrichan 3. Tarinee Chaiwong 4. Marutpong Panya 5. Nophawan Bunchu |
| Title | Species identification of forensically important fly larvaeby integrating morphological characteristics and protein profiles |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2563 |
| Journal Title | Songklanakarin Journal of Science and Technology |
| Journal Vol. | 42 |
| Journal No. | 6 |
| Page no. | 1215-1220 |
| Keyword | excretory and secretory (ES), protein profile, third stage larva, identification, flies |
| URL Website | https://rdo.psu.ac.th/sjstweb/index.php |
| ISSN | 0125-3395 |
| Abstract | The correct identification of fly species has a crucial role in accurately performed forensic investigations. Speciesidentification of fly larvae by observing only morphological characteristics is difficult and less accurate. Therefore, the objectiveof this study was to develop an alternative tool for identifying fly larvae by integrating morphological characteristics and proteinexpression profiles. Excretory-secretory (ES) protein profiles from third stage larvae of five fly species were evaluated todifferentiate these species, including Chrysomya megacephala, Achoetandrus rufifacies, Lucilia cuprina, Musca domestica, andBoettcherisca nathani, based on the SDS-PAGE technique. They were also assessed for morphological characteristics. Theresults showed that different protein patterns in ES products and morphological characteristics were observed among these flyspecies. A novel dichotomous key for identification of fly larvae was developed by combining unique patterns of ES proteinprofiles with morphological characteristics. However, studies including other fly species should be pursued. |
