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Short ITS DNA barcode effectivelydistinguishes the medicinal plants Cyclea barbata |
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| รหัสดีโอไอ | |
| Creator | 1. Kun Silprasit 2. Sirikul Thummajitsakul |
| Title | Short ITS DNA barcode effectivelydistinguishes the medicinal plants Cyclea barbata |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2563 |
| Journal Title | Songklanakarin Journal of Science and Technology |
| Journal Vol. | 42 |
| Journal No. | 6 |
| Page no. | 1197-1206 |
| Keyword | moonseed family, phylogenic tree, DNA barcode, morphology, Cyclea barbata |
| URL Website | https://rdo.psu.ac.th/sjstweb/index.php |
| ISSN | 0125-3395 |
| Abstract | Cyclea barbata has been reported as traditional food and medicinal plant. Although its morphology resemblesCissampelos pareira, Cyclea polypetala and Stephania japonica very closely, chemical compositions of them are significantlydifferent. Here we showed the capability of a short ITS region (277 nucleotides) for C. barbata identification. The partial ITS andrbcL regions from 14 samples, collected in many areas of Thailand, were amplified. The results after DNA blast on NCBIshowed that all short ITS sequences closely related to C. barbata. The phylogenic analysis from the ITS and combined ITS/rbcLdata by maximum parsimony and maximum likelihood methods showed that all samples was recognized into C. barbata. Theresults of DNA analysis were consistent with morphological identification. Our findings indicated that this short ITS region wasable to distinguish C. barbata from their adulterants. This in turn can reduce cost and time for C. barbata identification usingDNA barcode. |
