A green and effective method using oils to remove chlorophyll fromChromolaena odorata (L.) R.M. King & H. Rob
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Creator 1. Suppalak Phaisan
2. Gorawit Yusakul
3. Attapon Sakdamas
4. Nattapon Taluengjit
5. Seiichi Sakamoto
6. Waraporn Putalun
Title A green and effective method using oils to remove chlorophyll fromChromolaena odorata (L.) R.M. King & H. Rob
Publisher Research and Development Office, Prince of Songkla University
Publication Year 2563
Journal Title Songklanakarin Journal of Science and Technology
Journal Vol. 42
Journal No. 5
Page no. 1084-1090
Keyword Chromolaena odorata, chlorophyll removal, oil, antioxidant, flavonoids
URL Website https://rdo.psu.ac.th/sjstweb/index.php
ISSN 0125-3395
Abstract Chromolaena odorata (L.) R.M. King & H. Rob has excellent wound healing effects. Therefore, it is useful forcosmeceutical purposes; however, the dark-greenish appearance of its extract limits its attractiveness for product development.This study aimed to develop an oil-based system for chlorophyll removal. Dried ethanolic extracts were dissolved in differentaqueous EtOH solvents, and then the chlorophyll was partitioned to the oil phase. The efficiency of chlorophyll removal wasmonitored using a spectrophotometer. The recoveries of flavonoid and phenolic contents and the antioxidant activity weredetermined after the treatment. When the solutions of extract were prepared in 25% (v/v) - 75% (v/v) EtOH in water, the efficacyof chlorophyll removal by oil was higher than 85%. A higher concentration of EtOH resulted in a lower chlorophyll removalefficiency; however, the recoveries of phenolic and flavonoid contents and antioxidant activity improved. Palm oil showedefficiency higher than hexane for chlorophyll removal and high recoveries of the beneficial phytochemicals. This system can beapplied in the fields of natural products-based health product development and in phytochemical studies.
Songklanakarin Journal of Science and Technology (SJST)

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