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Population analysis of Epipactis flava Seidenf. in Thailandusing SRAP and RAPD markers |
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| รหัสดีโอไอ | |
| Creator | 1. Waroon Suwankitti 2. Sirinat Wankaew 3. Boonsita La-ongdet 4. Surin Peyachoknagul 5. Somjit Homchan 6. Anupan Kongbangkerd 7. Maliwan Nakkuntod |
| Title | Population analysis of Epipactis flava Seidenf. in Thailandusing SRAP and RAPD markers |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2563 |
| Journal Title | Songklanakarin Journal of Science and Technology |
| Journal Vol. | 42 |
| Journal No. | 5 |
| Page no. | 1065-1070 |
| Keyword | Epipactis flava, rheophytic orchid, DNA marker, diversity, population |
| URL Website | https://rdo.psu.ac.th/sjstweb/index.php |
| ISSN | 0125-3395 |
| Abstract | Epipactis flava Seidenf. is the only rheophytic orchid found in Thailand. Specific habitat requirements and environmental changes of the growing area have now resulted in E. flava becoming an endangered species and appropriatemanagement protocol is essential. Genetic diversity and population structure of E. flava were evaluated using RAPD and SRAPmarkers. Nine RAPD primers were selected with 95.93% polymorphism and eight SRAP primer combinations showing 92.29%polymorphism. Nei's genetic diversity (H) within populations from RAPD and SRAP was 0.2303 and 0.2016 and the Shannondiversity index (I) was 0.3380 and 0.2970, respectively. Among populations, H (0.3450 and 0.3237) and I (0.5156 and 0.4852)were also surveyed. Overall genetic diversity between and within populations was high. Results suggest that in situ conservationtogether with the introduction of plants from different locations is recommended to increase genetic variation. |
