Effects of culture medium and lighting condition on induction ofconidiation in Cercospora citrullina
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Creator 1. Kathrine Xin Yee Tan
2. Azlinda Binti Ibrahim
3. Hideyuki Nagao
Title Effects of culture medium and lighting condition on induction ofconidiation in Cercospora citrullina
Publisher Research and Development Office, Prince of Songkla University
Publication Year 2563
Journal Title Songklanakarin Journal of Science and Technology
Journal Vol. 42
Journal No. 4
Page no. 935-940
Keyword Cercospora, conidia, medium, lighting conditions, inoculation
URL Website https://rdo.psu.ac.th/sjstweb/index.php
ISSN 0125-3395
Abstract Cercospora citrullina, isolated from pumpkin plant (Cucurbita maxima), initially could not produce conidia whencultured on potato dextrose agar, but was able to produce conidia when cultured on oatmeal agar, oat grain agar, wheat grain agarand barley grain agar. C. citrullina also produced conidia when cultured in substrates such as oat grain, wheat grain, barley grainand Trichosanthes sp. dried leaves. Trichosanthes sp. dried leaves induced the highest conidia production (4.81 ? 103conidia /ml) while oatmeal agar and barley grain agar yielded the best result among the agar media by inducing 1.23 ? 103conidia / mleach. Overall, continuous darkness condition was better than other lighting conditions (12 h near UV black light plus 12 hdarkness condition; and 12 h fluorescent white light plus 12 h darkness lighting condition). Lastly, inoculation of C. citrullinaonto cucurbit plants was successfully conducted by sandwiching inoculum between leaves and plastic cards.
Songklanakarin Journal of Science and Technology (SJST)

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