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Growth profile and partial characterization of bacteriocin produced by Lactobacillus plantarum ATM11 isolated from slaughterhouse soil |
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| รหัสดีโอไอ | |
| Creator | 1. Alagu Thirumurugan 2. Rajaguru Sheela 3. Amaresh Kumar Singh |
| Title | Growth profile and partial characterization of bacteriocin produced by Lactobacillus plantarum ATM11 isolated from slaughterhouse soil |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2562 |
| Journal Title | Songklanakarin Journal of Science and Technology |
| Journal Vol. | 41 |
| Journal No. | 1 |
| Page no. | 37-44 |
| Keyword | bacteriocin, biochemical tests, 16S rRNA sequencing, L. plantarum ATM11, growth profile, physicochemical characterization |
| URL Website | http://rdo.psu.ac.th/sjstweb/index.php |
| ISSN | 0125-3395 |
| Abstract | In this study, we report on the growth profile and partial characterization of a bacteriocin produced by Lactobacillus plantarum ATM11 isolated from goat slaughterhouse soil. Among eight bacteriocin-producing bacteria from 25 Lactobacilli isolates of goat slaughterhouse soil, the higher-yielding bacteria were selected and identified as Lactobacillus plantarum based on the morphology, biochemical characteristics, and partial 16S rRNA gene sequence. The physico-chemical condition effects of temperature, pH, enzymes, and detergents of bacteriocin activity were studied. The optimum temperature for bacteriocin activity was found to be 60 ?C. The optimum pH for bacteriocin activity was found to be in the range of 5.0-6.0. Detergents such as sodium dodecyl sulphate (SDS), Triton X-100, and ethylene diamine tetra-acetic acid (EDTA) were influenced the bacteriocin activity, whereas urea inhibited the activity. Asparaginase and ?-amylase enzymes influenced the activity, whereas protease, proteinase K, and lysozyme inhibited bacteriocin activity. The present study revealed that the action mode of the produced bacteriocin has a bacteriostatic effect against Bacillus cereus MTCC 1272 and Micrococcus leuteus MTCC 2987. Thus, it can be used as a bio-preservative agent for different foods in the future. |
