|
Genetic structure of Pakistani tomato accessionsbased on morphological traits and RAPD markers |
|---|---|
| รหัสดีโอไอ | |
| Creator | 1. Usman Ahmed 2. Dawood Ahmad 3. Abdullah Jalal 4. Hala Rajab 5. Syed Sartaj Alam 6. Muhammad Sayyar Khan |
| Title | Genetic structure of Pakistani tomato accessionsbased on morphological traits and RAPD markers |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2562 |
| Journal Title | Songklanakarin Journal of Science and Technology |
| Journal Vol. | 41 |
| Journal No. | 6 |
| Page no. | 1348-1355 |
| Keyword | tomato, RAPD, diversity, molecular markers, morphological traits |
| URL Website | http://rdo.psu.ac.th/sjstweb/index.php |
| ISSN | 0125-3395 |
| Abstract | Genetic variations among eight Pakistani tomato (Solanum lycopersicum L.) accessions were investigated usingmorphological traits and randomly amplified polymorphic DNA (RAPD) primers. Significant differences among accessions wereobserved for most of the quantitative and qualitative morphological traits. The RAPD-PCR assay amplified a total of 39 differentalleles with 33 polymorphic alleles resulting in an 84.25% rate of polymorphism among eight tomato accessions. The mostinformative primers were B06 and H05 by revealing the maximum number of bands in the germplasm. The dendrogram for themorphological traits classified the germplasm into a single larger cluster except for genotype 17880. The dendrogram constructedfor the molecular data clearly segregated the germplasm into two clusters. The accessions 17862 and 17870 were placed in thesmall cluster while the rest of the accessions were grouped together in the larger cluster. Genetic similarity ranged from 0.31 to0.83 with an average of 0.68 for all evaluated accessions. |
