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Effect of low-protein diets supplemented with methionineon growth performance, carcass characteristics, and expression of ANTand UCP genes in the breast muscle of Betong chickens (KU Line) |
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| รหัสดีโอไอ | |
| Creator | 1. Jiraporn Marayat 2. Somruthai Lertpimonpan 3. Choawit Rakangthong 4. Panwadee Sopannarath 5. Chaiyapoom Bunchasak 6. Wiriya Loongyai |
| Title | Effect of low-protein diets supplemented with methionineon growth performance, carcass characteristics, and expression of ANTand UCP genes in the breast muscle of Betong chickens (KU Line) |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2562 |
| Journal Title | Songklanakarin Journal of Science and Technology |
| Journal Vol. | 41 |
| Journal No. | 6 |
| Page no. | 1211-1218 |
| Keyword | adenine nucleotide translocase protein, uncoupling protein, Betong Chicken (KU line), low protein diets, methionine supplementation |
| URL Website | http://rdo.psu.ac.th/sjstweb/index.php |
| ISSN | 0125-3395 |
| Abstract | We investigated the effects of low-protein diets supplemented with methionine on the growth performance of Betongchickens (KU-Line) from 4 to 18 weeks. A total of 192 four-week-old male Betong chickens were divided into three groups:high-protein diet group (high crude protein [CP]), low protein diet group (low CP), and low-protein diet group supplementedwith methionine (low CP+Met). We found no significant effect of protein levels on the body weight gain and average daily gain.Betong chickens fed a low-CP diet showed the highest feed intake (P<0.01) and protein intake (P<0.05), while birds fed the lowCP+Met diet had the highest methionine intake (P<0.05). The addition of methionine to the low CP diet resulted in a significantlyimproved feed conversion ratio compared to the low CP and the high CP diet groups (P<0.01). Moreover, the feed cost wasreduced in the low CP+Met diet compared to the high CP diet (P>0.05). At the end of the experiment, two birds per replicatewere randomly selected and slaughtered. The carcass weights were not significantly different between the treatments. Theabdominal fat yield decreased in the treatment with methionine supplementation (P<0.05) at 12 weeks. Breast muscle sampleswere collected for total RNA extraction. The cDNA was amplified using primers specific for ANT and UCP gene expression andanalyzed using real-time PCR. At 12 weeks, mRNA ANT expression was significantly higher in the muscle of birds fed dietssupplemented with methionine. The highest UCP expression was obtained from the low CP diet group, which also resulted in theworst feed conversion ratio at 18 weeks. Our results indicated that methionine supplementation in low CP diets improved growthperformance, reduced feed costs, and influenced the expression of ANT and UCP in the muscle. |
