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Precise discrimination of lymphoproliferative disorders with combinedTCRG and TCRB: Japanese series |
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| รหัสดีโอไอ | |
| Creator | 1. Setsuko Miyanishi 2. Kayo Takeoka 3. Seiji Kawano 4. Pimpicha Patmasiriwat 5. Sumana Dakeng |
| Title | Precise discrimination of lymphoproliferative disorders with combinedTCRG and TCRB: Japanese series |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2562 |
| Journal Title | Songklanakarin Journal of Science and Technology |
| Journal Vol. | 41 |
| Journal No. | 5 |
| Page no. | 1101-1109 |
| Keyword | TCRG, TCRB, ATLL, mature T-cell disorder, lymphoproliferation |
| URL Website | http://rdo.psu.ac.th/sjstweb/index.php |
| ISSN | 0125-3395 |
| Abstract | TCR gene rearrangement detection has proven to be a useful diagnostic method. In this work, 88 T-celllymphoproliferative disorders and 45 non-T-cell proliferation in Japanese patients were studied for rearrangement of TCRG andTCRB using PCR and multiplex PCR. The sensitivity and specificity of TCRB assay in mature T-cell proliferation were 98.2%and 90.9%, respectively. The sensitivity and specificity of TCRG were 84.0% and 73.3%, respectively. The combination ofrearranged TCRG and TCRB analysis provides an extremely high sensitivity for the diagnosis of mature T-cell proliferativedisorders. All patients in this group exhibited rearrangement of either TCRG or TCRB or both TCRG and TCRB. All cases of Blymphoid malignancies and reactive lymphoid cases tested for TCRB-DJ gave complete negative results, thus providing analternative discrimination from CD3(+) and T-cell leukemia/lymphoma. Although 25% of B-cell non-Hodgkin lymphoma (BNHL) cases revealed TCRG rearrangement, all B-NHL cases gave negative TCRB rearrangement. In summary, PCR-basedcombination analysis of TCRG and TCRB rearrangement is a powerful diagnostic tool which also provides accuratediscrimination for each type of lymphoproliferative disorder |
