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Porcine cumulus oocyte complexes (pCOCs)as biological model for determination on in vitro cytotoxicof cadmium and copper assessment |
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| รหัสดีโอไอ | |
| Creator | 1. Waramanee Pongsawat 2. Mayuva Youngsabanant |
| Title | Porcine cumulus oocyte complexes (pCOCs)as biological model for determination on in vitro cytotoxicof cadmium and copper assessment |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2562 |
| Journal Title | Songklanakarin Journal of Science and Technology |
| Journal Vol. | 41 |
| Journal No. | 5 |
| Page no. | 1029-1036 |
| Keyword | cadmium, copper, cytotoxicity, porcine cumulus oocyte complexes (pCOCs), electron microscopy |
| URL Website | http://rdo.psu.ac.th/sjstweb/index.php |
| ISSN | 0125-3395 |
| Abstract | We demonstrated the potential of primary porcine cumulus oocyte complexes (pCOCs) used as a biological model fordetermination of in vitro toxicity of cadmium and copper. After treatment at a concentration of 10-4 M of cadmium and copperfor 44 h, the surrounding cumulus cells (CCs) stopped the expansion process and the pCOCs did not undergo maturation in eithertreatment. Compared to the control, degradation of the pCOCs was observed as well as a number of vacuoles associated withmetal accumulation. Cumulus cells and zona-pellucida revealed a rough surface with numerous knobs. The cumulus cells showeda loss of cell margin and signs of cytoplasmic organelle damage. The percent adsorption rate of cadmium and copper frominductively coupled plasma were 5.65 and 6.85, respectively, and there was no significant difference between treatment groups(P>0.05). This finding is valuable for future cell technology research in medical and in vitro cytotoxicity studies. |
