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Decolorization of synthetic dyes using partially purified peroxidase from green cabbage (Brassica oleracea) |
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| รหัสดีโอไอ | |
| Creator | 1. Cliff C. Victor 2. Kingsley Ozioma Omeje 3. Arinze Linus Ezugwu 4. Sabinus Oscar Onyebuchi Eze 5. Ferdinand Chiemeka Chilaka 6. Ikechukwu Noel Emmanuel Onwurah 7. Pius Oziri Ukoha |
| Title | Decolorization of synthetic dyes using partially purified peroxidase from green cabbage (Brassica oleracea) |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2562 |
| Journal Title | Songklanakarin Journal of Science and Technology |
| Journal Vol. | 41 |
| Journal No. | 4 |
| Page no. | 783-787 |
| Keyword | decolorization, green cabbage, peroxidase, synthetic dyes |
| URL Website | http://rdo.psu.ac.th/sjstweb/index.php |
| ISSN | 0125-3395 |
| Abstract | Peroxidase was extracted from cabbage and was purified 13.14 fold by a three-step process of ammonium sulphate precipitation, dialysis, and gel filtration with a yield of 9.44. The partially purified peroxidase had maximal activity at pH 5.0 and temperature 45?C. Michaelis constant (Km) and Maximum velocity (Vmax) obtained from Lineweaver-Burk plot of initial velocity data at different concentrations of H2O2 and O-dianisidine were 3.68Mm/ 37.04U and 9.89mM/ 28.57U, respectively. The partially purified peroxidase from cabbage decolorized many synthetic Azo and Vat dyes, with Azo tryptan blue having highest decolorization of 88.62% while vat orange II showed 12% decolorization, after contact time of one hour. This suggests that peroxidase from cabbage has the potential to decolorize synthetic dyes. |
