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Antioxidation and wound healing activity of a recombinant proteinfrom Penaeus monodon |
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| รหัสดีโอไอ | |
| Creator | 1. Ajchara Kaewjurat 2. Jasadee Kaewsrichan 3. Amornrat Phongdara |
| Title | Antioxidation and wound healing activity of a recombinant proteinfrom Penaeus monodon |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2561 |
| Journal Title | Songklanakarin Journal of Science and Technology |
| Journal Vol. | 40 |
| Journal No. | 4 |
| Page no. | 953 |
| Keyword | Penaeus monodon, Pm-fortilin, cell migration, wound healing, antioxidant |
| URL Website | http://rdo.psu.ac.th/sjstweb/index.php |
| ISSN | 0125-3395 |
| Abstract | Pm-fortilin is a recombinant protein of Penaeus monodon expressed in Escherichia coli. Activities regarding antioxidationand wound healing of the purified Pm-fortilin were investigated in vitro using normal fibroblast cell line L929. Relativeviabilities of the cells were determined after exposure to either hydrogen peroxide (0.5 mmol/l for 4 h) or UV radiation (366 nmfor 20 min). Significantly decreased viability was observed for the UV exposed cells compared with those incubated with theperoxide. Interestingly, proliferation of the stress-induced cells was improved by Pm-fortilin treatment. The protein's curingeffect was dose dependent. The scratch method was used in the wound healing test on L929 cells. The injured cells distinctivelymigrated if pre-incubation with Pm-fortilin was carried out. However, such migration ability was compromised if the injuredcells were continually in contact with Pm-fortilin. The transcriptions of sydecan-2, dkk1, ?-catenin, lrp6, and bmi1 genes inMG63 and HOS cancerous cells were down-regulated following incubation with Pm-fortilin for 5 days compared to thosetranscribed by L929 cells. The results suggested that Pm-fortilin might be non-oncogenic and is premised for use as a protectiveor repair agent in topical pharmaceutical products. |
