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Species identification of economic bamboosin the genus Dendrocalamus using SCAR and multiplex PCR |
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| รหัสดีโอไอ | |
| Creator | 1. Achariya Rangsiruji 2. Sutheewan Binchai 3. Onanong Pringsulaka |
| Title | Species identification of economic bamboosin the genus Dendrocalamus using SCAR and multiplex PCR |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2561 |
| Journal Title | Songklanakarin Journal of Science and Technology |
| Journal Vol. | 40 |
| Journal No. | 3 |
| Page no. | 640 |
| Keyword | bamboo, Dendrocalamus, multiplex PCR, RAPD, SCAR |
| URL Website | http://rdo.psu.ac.th/sjstweb/index.php |
| ISSN | 0125-3395 |
| Abstract | Taxonomic and systematic studies of bamboos are traditionally based on floral morphology, but this can lead todifficulties in identification because of the irregular reproductive cycle of the bamboos. To overcome such problems severalmolecular-marker approaches have been used. In this study, eight species of the woody bamboos belonging to the genusDendrocalamus were employed. For each species, DNA samples of 20 individual plants from different localities were isolatedand then pooled to make eight bulks of DNA. Fifty RAPD primers were used to screen all bulked DNA samples. Only fiveprimers yielded consistent and reproducible RAPD band patterns across all 160 individuals. The amplicons were present amongfive species of Dendrocalamus, but were absent in the other three species. They were cloned, sequenced and subsequently, fivepairs of SCAR primers were designed. All SCAR primers were combined in multiplex PCR reactions to unequivocallydiscriminate five species of Dendrocalamus. |
