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Genotype of Javanese backyard waterfowl based onantiviral myxovirus gene |
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| รหัสดีโอไอ | |
| Creator | 1. R. Susanti 2. Fidia Fibriana 3. Fitri Arum Sasi |
| Title | Genotype of Javanese backyard waterfowl based onantiviral myxovirus gene |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2561 |
| Journal Title | Songklanakarin Journal of Science and Technology |
| Journal Vol. | 40 |
| Journal No. | 3 |
| Page no. | 498 |
| Keyword | domestic waterfowl, genotyping, Mx gene, PCR-RFLP |
| URL Website | http://rdo.psu.ac.th/sjstweb/index.php |
| ISSN | 0125-3395 |
| Abstract | Myxovirus (Mx) proteins are essential antiviral protein components induced by interferon in many species. This studyaims to determine the genotype of 72 Javanese backyard waterfowl based on the Mx gene. Mx gene was amplified using PCRRFLPtechnique by primers NE-F2/F and NE-R2/R. The expected 100bp PCR product was visualized by 1.2% high-resolutionMetaPhor? Agarose. To determine the genotype of Mx gene, the PCR fragment was cut by RsaI. The results showed that allMx-- samples generated 73bp and 27bp fragments, indicating that all waterfowl species were (GG genotype) and showedsusceptibility to the avian influenza virus infection. The susceptible species are difficult to be identified based on its morphologicalappearance since waterfowl do not show any symptoms of illness. Therefore, Mx gene genotyping has the potential forbreed selection to obtain healthy and resistant waterfowl both phenotype and genotype. Targeting the Mx gene is a potentialapproach for the development of avian influenza-resistant poultry. |
