|
In vitro propagation through transverse thin cell layer (tTCL) culturesystem of lady's slipper orchid: Paphiopedilum callosum var. sublaeve |
|---|---|
| รหัสดีโอไอ | |
| Creator | 1. Nararatn Wattanapan 2. Charassri Nualsri 3. Upatham Meesawat1 |
| Title | In vitro propagation through transverse thin cell layer (tTCL) culturesystem of lady's slipper orchid: Paphiopedilum callosum var. sublaeve |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2561 |
| Journal Title | Songklanakarin Journal of Science and Technology |
| Journal Vol. | 40 |
| Journal No. | 2 |
| Page no. | 306 |
| Keyword | RAPD, somaclonal, transvers thin cell layer, Paphiopedilum |
| URL Website | http://rdo.psu.ac.th/sjstweb/index.php |
| ISSN | 0125-3395 |
| Abstract | Paphiopedilum callosum var. sublaeve, a lady's slipper orchid native to southern Thailand, is protected under CITES(appendix I) due to habitat destruction and over-collection. The novel micropropagation technique for this endemic species wasinvestigated. A 2-week preculture of seeds in distilled water before transfer to 1/2MS solid medium was the optimal conditions topromote seed germination in vitro. Shoot tip-derived transverse thin cell layer (tTCL) explants cultured on MVW solid mediumcontaining 1.0 mg/L TDZ for 8 weeks provided the highest percentage of regenerated protocorm-like bodies (46.67?6.67), shootformation (40.00?5.16), root formation (30.00?12.38) and survival rate (70.00?4.47). The acclimatized plantlets potted insphagnum moss in the greenhouse grew well at 80% survival rate. No genetic variation was detected between the regeneratedplantlets and their own mother plants based on RAPD marker. |
