|
Quantitative analysis of a phenanthrene from Eulophia species by TLC-image analysis method |
|---|---|
| รหัสดีโอไอ | |
| Creator | 1. Kanokwan Changwichit 2. Corine Girard 3. Prapapan Temkitthawon 4. Nantaka Khorana 5. Kornkanok Ingkaninan |
| Title | Quantitative analysis of a phenanthrene from Eulophia species by TLC-image analysis method |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2561 |
| Journal Title | Songklanakarin Journal of Science and Technology |
| Journal Vol. | 40 |
| Journal No. | 6 |
| Page no. | 1324-1328, |
| Keyword | TLC-image analysis, phenanthrenes, PDE5 inhibitors, Eulophia macrobulbon |
| URL Website | http://rdo.psu.ac.th/sjstweb/index.php |
| ISSN | 0125-3395 |
| Abstract | A phenanthrene constituent in Eulophia macrobulbon (E.C.Parish & Rchb.f.) Hook.f., 1-(4'-hydroxybenzyl)-4,8- dimethoxyphenanthrene-2,7-diol (1) was previously reported as a phosphodiesterase 5 inhibitor. A simple and rapid thin layer chromatographic (TLC)-image analysis method was developed to quantify this compound in plant samples. Chromatographic separation of the phenanthrene was completed on silica gel TLC using dichloromethanemethanol (96:4, v/v) as a mobile phase. Image analysis of the scanned TLC plate was performed by Photoshop CS6 to quantify the amount of phenanthrenes. The area under the spot corresponding to 1 was integrated and used for quantification. The method was validated by the detection limit, calibration curve, repeatability, reproducibility, and recovery. The results showed that our digital imaging method was applicable for quantification of 1 on TLC. The method was convenient, efficient, and moderately accurate for the quantitative analysis of 1 in plant samples. |
