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In vitro adhesion property and competition against enteropathogens ofLactobacillus strains isolated from Thai infants |
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| รหัสดีโอไอ | |
| Creator | 1. Kwannan Nantavisai 2. Srisombat Puttikamonkul 3. Kruawan Chotelersak 4. Malai Taweechotipatr |
| Title | In vitro adhesion property and competition against enteropathogens ofLactobacillus strains isolated from Thai infants |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2561 |
| Journal Title | Songklanakarin Journal of Science and Technology |
| Journal Vol. | 40 |
| Journal No. | 1 |
| Page no. | 69 |
| Keyword | probiotics, Lactobacillus, Caco-2 cell, adhesion, competition, Salmonella Typhi, Shigella dysenteriae |
| URL Website | http://rdo.psu.ac.th/sjstweb/index.php |
| ISSN | 0125-3395 |
| Abstract | Adhesion to the intestinal epithelium is considered to be one of the selection criteria for probiotics strain. In this study, theadhesion of four different Lactobacillus strains with potential probiotics properties, i.e. L. paracasei MSMC39-1, L. caseiMSMC39-3, L. salivarius MSMC105-3 and L. plantarum MSMC171-1, was studied using Caco-2 cell line as an in vitro modelfor intestinal epithelium. Among four different Lactobacillus strains, L. salivarius MSMC105-3 was the most adhesive strainshowing about 3.5 percent of adhesion index. Thus, this strain was selected to examine for its ability to inhibit the adhesion ofpathogenic Salmonella Typhi DMST5784 and Shigella dysenteriae DMST15111 to Caco-2 cells. The results showed that L.salivarius MSMC105-3 whole cell and its cell-free culture supernatant could inhibit the adhesion of pathogens. The results fromthis study indicated that both L. salivarius MSMC105-3 itself and its substances secreted into culture supernatant had the abilityto reduce the adhesion of enteropathogens to Caco-2 cells. |
