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In silico analysis of cysteine cathepsins identified fromthe transcriptome profile of blunt snout bream(Megalobrama amblycephala) |
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| รหัสดีโอไอ | |
| Creator | 1. Ngoc Tuan Tran 2. Wei-Min Wang |
| Title | In silico analysis of cysteine cathepsins identified fromthe transcriptome profile of blunt snout bream(Megalobrama amblycephala) |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2561 |
| Journal Title | Songklanakarin Journal of Science and Technology |
| Journal Vol. | 40 |
| Journal No. | 5 |
| Page no. | 1017-1025 |
| Keyword | cysteine cathepsin, blunt snout bream, in silico, homology modelling, codon usage |
| URL Website | http://rdo.psu.ac.th/sjstweb/index.php |
| ISSN | 0125-3395 |
| Abstract | Cysteine cathepsins are described as lysosomal proteases with housekeeping and highly specialised functions in manyorganisms. In this study, cysteine cathepsins were identified from the transcriptome profile of blunt snout bream (Megalobramaamblycephala). A total of 41 cysteine cathepsin-like sequences were found and divided into groups of cathepsin (Cts): B, C, F, H,K, L, S, and Z. Twenty-nine of these sequences contained coding sequences, encoding 92-473 amino acids, which exhibited thehighest (78%-95%) homology to the counterparts from zebrafish (Danio rerio). Multiple sequence alignment of the amino acidsshowed highly conserved domains among the cysteine cathepsins of M. amblycephala to its homologs. The putative proteins,including maCtsb1, maCtsc1, maCtsf2, maCtsh1, maCtsk5, maCtsl5, maCtss1, and maCtsz5 were characterised and structuredusing in silico methods. Additionally, codon usage of full length open reading frame sequences of these cathepsins was analysed.This study provided basic information for further studies on the specific functions of cysteine cathepsins of M. amblycephala inthe future. |
