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Solid-phase extraction for determination of caffeine and itsdimethylxanthine metabolites in rat urine using high performanceliquid chromatography and its application |
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| รหัสดีโอไอ | |
| Creator | 1. Keson Trakunram 2. Benjamas Janchawee 3. Pritsana Raungrut 4. Sathaporn Prutipanlai |
| Title | Solid-phase extraction for determination of caffeine and itsdimethylxanthine metabolites in rat urine using high performanceliquid chromatography and its application |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2561 |
| Journal Title | Songklanakarin Journal of Science and Technology |
| Journal Vol. | 40 |
| Journal No. | 4 |
| Page no. | 759 |
| Keyword | caffeine, SPE, urine, rat |
| URL Website | http://rdo.psu.ac.th/sjstweb/index.php |
| ISSN | 0125-3395 |
| Abstract | A solid-phase extraction (SPE) method for use with high performance liquid chromatography to measure caffeine (CF)and its metabolites in rat urine was developed and validated. Oasis HLB cartridges were used and washed with water, 1% aceticacid: 3% methanol-water (0.5:99.5 v/v), and 1% ammonium hydroxide: 3% methanol-water (0.5:99.5 v/v), respectively, andeluted with methanol:acetronitrile (80:20 v/v). Method validation showed good linearity (r>0.9990) over all calibration rangesfor CF and its metabolites. The method was precise (1.017.6%RSD) and accurate (?13.8(+) 16.0%DEV). The SPE methodresulted in a high recovery for all analytes (72.9?98.9%). The lower limits of quantification were 0.1 ?g/ml (PX), 0.25 ?g/ml (CFand theophylline), and 0.5 ?g/ml (theobromine and 1,3,7-trimethyluric acid). This method was successfully applied to determinethe urinary profiles of CF and its metabolites, and the caffeine metabolic ratios in rats receiving a single oral dose of CF. |
