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Phytoalexin production of lettuce (Lactuca sativa L.) grown in hydroponicsand its in vitro inhibitory effect on plant pathogenic fungi |
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| รหัสดีโอไอ | |
| Creator | 1. Chulalak Talubnak 2. Nonglak Parinthawong 3. Tanimnun Jaenaksorn |
| Title | Phytoalexin production of lettuce (Lactuca sativa L.) grown in hydroponicsand its in vitro inhibitory effect on plant pathogenic fungi |
| Publisher | Research and Development Office, Prince of Songkla University |
| Publication Year | 2560 |
| Journal Title | Songklanakarin Journal of Science and Technology (SJST) |
| Journal Vol. | 39 |
| Journal No. | 5 |
| Page no. | 633 |
| Keyword | phytoalexin,biotic elicitor,abiotic elicitor,lettuce,hydroponics |
| ISSN | 0125-3395 |
| Abstract | A series of experiments were conducted to investigate phytoalexin production from five varieties of lettuce in hydroponicsusing abiotic (2.5, 5% CuSO4; 0.5, 1% AgNO3) and biotic elicitors (non-pathogenic Pythium sp.) at different plant ages.It showed that phytoalexin was successfully induced in tested lettuce grown in hydroponics after elicitation with abioticelicitors throughout the trial. Phytoalexin showed yellow fluorescent spot under 365 nm UV light with Rf 0.45-0.48 and clearinhibition zone where Aspergillus niger failed to develop on TLC plate. For biotic elicitors, no yellow fluorescent spot onTLC plate was observed from tested lettuce varieties however inhibition zone at Rf 0.9 was detected at 8-9 weeks from red oak,green oak, and red coral. Moreover, crude extract of lettuce elicited with abiotic elicitors possessed in vitro antifungal activityagainst C. gloeosporioides, C. lunata, F. oxysporum, and P. aphanidermatum probably due to phytoalexin (lettucenin A) inthe extract. |
